Production of Lytic Exoenzymes in Casamino Acids Media by Myxococcus virescens

The production of proteolytic enzymes and enzymes active on autoclaved aerobacter cells by Myxococcus virescens, strain B2, was studied In Casamino acids media. By modification of the N III–B media of Norén to a higher concentration of casamino acids and to a lower content of sodium chloride, the maximal production of lytic exoenzymes could be increased at least four times. Too high a concentration of casamino acids (more than 17 g/1) and sodium chloride (30 mM), as well as too low a concentration of sodium chloride, inhibited the enzyme production.