Key result
Addition of S-nitroso-N-acetyl penicillamine (SNAP) to RAW264-7 cells enhanced the expression of Mn-SOD and CuZn-SOD, suggesting SOD expression may protect cells from NO toxicity.
Why the study?
Does S-nitroso-N-acetylpenicillamine (SNAP) enhance the expression of superoxide dismutases mRNA in the murine macrophage cell line RAW264-7?
Does S-nitroso-N-acetylpenicillamine (SNAP) enhance the expression of superoxide dismutases mRNA in the murine macrophage cell line RAW264-7?
Nitric oxide-releasing agents like SNAP enhance the expression of superoxide dismutases in murine macrophages, suggesting a protective mechanism against NO toxicity.
SNAP effects in murine macrophages warrant no clinical action; leaves open whether SOD induction protects against NO toxicity in human disease.
Murine interferon-gamma (IFN-gamma) stimulates the murine macrophage tumour cell line RAW264-7 to produce nitric oxide (NO). IFN-gamma induces expression of inducible NO synthase (iNOS), manganese superoxide dismutase (Mn-SOD) and copper zinc SOD (CuZn-SOD) in these cells. To investigate the mechanism of induction of SOD expression, we added S-nitroso-N-acetyl penicillamine (SNAP) to RAW264-7 cells. SNAP enhanced the expression of Mn-SOD and CuZn-SOD. These results suggest that when producing NO, RAW264-7 cells express SOD that might protect them from NO toxicity.
No takes yet. Share an insight, caveat, or question.
Sano et al. (1997) studied Murine macrophage cell line RAW264-7. S-nitroso-N-acetyl penicillamine (SNAP) was evaluated on Expression of Mn-SOD and CuZn-SOD mRNA. Addition of S-nitroso-N-acetyl penicillamine (SNAP) to RAW264-7 cells enhanced the expression of Mn-SOD and CuZn-SOD, suggesting SOD expression may protect cells from NO toxicity.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: