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Objective: A simple and fast high performance liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) method has been developed for the analysis of brassinosteroids (BRs) in plants without derivatization. Materials: The BRs (including castasterone, 24-epicastasterone, brassinolide and 24-epibrassinolide) have been extracted with ice cold 80% aqueous methanol solution. Method: Five different purification strategies have been tested for the purification and enrichment of BRs. Conclusion: This analytical method was sensitive, reliable, rapid and applicable to trace analysis in complex plant samples.
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Li et al. (2017) studied this question.
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