The addition of penicillin (50u/ml) to the cells of Corynebacterium alkanolyticum No. 314 growing on n-paraffins medium at the logarithmic growth phase was the most suitable for the extracellular accumulation of L-glutamic acid and the excretion of phospholipids. The relation between the extracellular accumulation L-glutamic acid and the excretion of phospholipids-in the presence of penicillin was very close and specific. The kinds of phospholipids excreted and their fatty acid components were the same as those of intracellular phospholipids.
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Kikuchi et al. (1973) studied this question.