Key result
Exposure to the calcium channel blocker D-600 at 7 degrees C paralyses frog skeletal muscle fibres, an effect that is absent at 22 degrees C and reversible upon warming.
Population
Single skeletal muscle fibres of the frog
Comparison
D-600 exposure at 7 degrees C and 22 degrees C vs null
Design
Preclinical
Authors
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Caution against extrapolating frog muscle paralysis to clinical calcium channel blocker use; leaves open temperature effects on excitation-contraction coupling.
D-600 paralyses frog skeletal muscle fibres at 7 degrees C but not at 22 degrees C, suggesting it disrupts the mechanism coupling electrical potential changes to calcium release.
Eisenberg et al. (1983) studied Frog skeletal muscle fibres. D-600 (methoxyverapamil) vs. 22 degrees C exposure was evaluated on Paralysis (inability to contract in response to electrical stimulation or K+). Exposure to the calcium channel blocker D-600 at 7 degrees C paralyses frog skeletal muscle fibres, an effect that is absent at 22 degrees C and reversible upon warming.
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