An enzyme preparation from 2,4-dichlorophenoxyacetate-grown Arthrobacter sp. converted cis,cis-2,4-dichloromuconate to chloromaleylacetate. The enzyme lactonizing the dichloromuconate to yield 2-chloro-4-carboxymethylene but-2-enolide was separated from the butenolide-delactonizing enzyme.
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Sharpee et al. (1973) studied this question.
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