MicroRNA expression profiles in familial hypertrophic cardiomyopathy with myosin-binding protein C3 (MYBPC3) gene mutations

Figures

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  Fig. 1. Echocardiography of FHCM patients showed abnormal ventricular septal thickness. a Patient 1, female, 27 years old. Echocardiography showed that the left ventricular myocardium showed asymmetric hypertrophy, and the thickest left ventricular wall was 2.38 cm. b Patient 2, male, 68 years old. Echocardiography showed that the left ventricular myocardium showed asymmetric hypertrophy, and the thickest left ventricular wall was 2.18 cm. c Patient 3, female, 84 years old. Echocardiography showed that the left ventricular myocardium showed asymmetric hypertrophy, and the thickest left ventricular wall was 3.36 cm

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  Fig. 2. Differentially expressed miRNAs in FHCM patients. a Scatter plots of differentially expressed miRNAs in FHCM and control groups. Green dots represent the significantly downregulated miRNAs, red dots represent the significantly upregulated miRNAs, and gray dots represent the non-significantly expressed miRNAs. Two oblique dashed lines divide up and down (2 times) and unchanged miRNAs. b Volcano plot of differentially expressed miRNAs in FHCM and control groups. The two vertical green lines represent upregulated (right) and downregulated (left) miRNAs, respectively, and the green parallel lines correspond to the p value. Green dots represent the significantly downregulated miRNAs, red dots represent the significantly upregulated miRNAs, and gray dots represent the non-significantly expressed miRNAs. c Hierarchical clustering plot shows that the significant differentially expressed miRNAs. (Test for FHCM patients, and Control for healthy group). Each column represents the expression profile of a tissue sample, and each row corresponds to a miRNA. Red means high expression level and green means lower levels

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  Fig. 3. Hsa-miR-208b-3p is upregulated in FHCM patients plasma. a The CPM values of has_miR-208b-3p in miRNA-seq analysis data. Green bar chart indicates the mean value of healthy group CPM value and red indicates FHCM group. b RT-qPCR validation result suggests hsa-miR-208b-3p is upregulated in the peripheral blood plasma of FHCM patients with MYBPC3 gene mutations

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  Fig. 4. Hsa-miR-208b-3p and its target genes network. a Venn plot show the intersection result of miR-208b-3p target genes predicted from Targetscan and miRdb. b miR-208b-3p and 79 target gene network. Yellow circle is hsa-miR-208b-3p, and blue circles is the targeted genes predicted by Targetscan and miRdb database

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  Fig. 5. Functional enrichment analysis result indicates the complex regulatory functions of miR-208b-3p in the MYBPC3 mutations FHCM patients. a GO analysis of miR-208b-3p. The red bars represent biological process terms, the green bars represent cellular components terms, and the blue bars represent molecular function terms. Horizontal axis means enrichment score (equal to − log10(p value)). Every bar means every enriched GO terms. b KEGG pathway analysis of miR-208b-3p. Horizontal axis means enrichment score (equal to − log10(p value)). Each bar indicates one pathway terms. c The schematic illustration of miR-208b-3p