Polyphenol Composition of Extract from Aerial Parts of Circaea Lutetiana L. and its Antioxidant and Anti-Inflammatory Activity in Vitro

The chemical composition and bioactivity of a water/methanol extract prepared from aerial parts of Circaea lutetiana were determined.HPLC-DAD-MS 3 analysis revealed the presence of 14 different compounds comprising phenolic acids, ellagitannins and flavonoids.Antioxidant assays showed the extract's strong scavenging activity towards DPPH (SC 50 33.1±3.1 μg/ml), O 2 -(SC50 4.0±2.3μg/ml) and H 2 O 2 (SC 50 below 2 μg/ml).Enzyme-based studies revealed that Circaea lutetiana extract inhibits the activity of hyaluronidase (IC 50 13.3±2.4μg/ml) and lipoxygenase (IC 50 44.7±1.4 μg/ml).These results support some traditional uses of Circaea lutetiana.