A Differential Medium for Semiselective Isolation of Xanthomonas campestris pv. vesicatoria and Other Cellulolytic Xanthomonads from Various Natural Sources

A semiselective medium (CMC-E) was more effective than Tween medium B agar or nutrient agar for recovery of Xanthomonas campestris pv. vesicatoria from the environment or plant material. The selectivity of CMC-E medium was from the sodium salt of high-viscosity carboxymethyl cellulose as the primary carbon source and gelatin as the sole source of nitrogen. Additional selectivity was from the incorporation of eosin Y, methylene blue, bacitracin, cephalexin, cycloheximide, 5-fluorouracil, neomycin sulfate, and tobramycin