Key result
Next generation sequencing identified suitable single nucleotide polymorphism markers in 11 candidate genes related to innate immunity and virus cell entry in two Cetacean species.
Observational
Identified genetic markers in cetaceans that may be useful for studying host genetic variation and susceptibility to morbillivirus infection.
Hypothesis-generating for cetacean morbillivirus susceptibility; leaves open validation and cross-species translation.
Morbilliviruses, such as Cetacean morbillivirus (CeMV) or Phocine distemper virus (PDV), represent a growing threat for marine mammals on both hemispheres. Because free-ranging animal populations strongly rely on natural resistance mechanisms, innate immunity-related genes and virus cell entry receptor genes may represent key factors involved in susceptibility to CeMV in Cetaceans. Using the next generation sequencing technology, we have sequenced 11 candidate genes in two model species, Stenella coeruleoalba and Phocoena phocoena. Suitable single nucleotide polymorphism markers of potential functional importance, located in genes coding for basigin (BSG, CD147), the signaling lymphocyte activating molecule (SLAMF1), the poliovirus-related receptor-4 (NECTIN4, PVRL4), toll-like receptors 3, 7, 8 (TLR3, TLR7, TLR8), natural resistance-associated macrophage protein (SLC11A1) and natural cytotoxicity triggering receptor 1 (NCR1), were identified in each model species, along with MHC-DQB haplotypes unique for each species. This set of molecular markers represents a potentially useful tool for studying host genetic variation and susceptibility to morbillivirus infection in Cetaceans as well as for studying functionally important genetic diversity of selected Cetacean populations.
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Stejskalová et al. (2017) conducted an observational in Morbillivirus infection susceptibility. Next generation sequencing of candidate genes was evaluated on Identification of single nucleotide polymorphism markers. Next generation sequencing identified suitable single nucleotide polymorphism markers in 11 candidate genes related to innate immunity and virus cell entry in two Cetacean species.
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