Bull spermatozoa were aged in vitro for periods of up to 9 days. Microspectro. photometric measurements were made of the nuclear DNA content of the sperm heads using ultraviolet absorption, acridine orange fluorescence, and Feulgen staining and of the nuclear histone content using fast green staining. The surface area of the sperm heads was calculated from linear measurements of head length and width.
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Blackshaw et al. (1972) studied this question.