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A continuous line of human breast carcinoma cells, HBT-3, was established in culture following collagenase treatment of a mucus producing adenocarcinoma. The cells are epithelioid in appearance, multiply rapidly, have a cloning efficiency of approximately 70%, and exhibit an abnormal karyotype with a mode of 66-69 chromosomes/cell and 3 markers.
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Bassin et al. (1972) studied this question.
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