The bone-resorbing activity of prostaglandin was assessed in neonatal mouse calvaria maintained in culture as well as in the bones of adult mice in vivo. In an in vitro45Ca-release bioassay, PGE2, the most active compound, produced a maximal stimulation of radiocalcium bone depletion of 89% versus 38% for PGF2α and 12% for PGA1. Daily intraperitoneal injection of 10 μg of 16,16-dimethyl-PGE2-methyl ester, a long-acting synthetic analog of PGE2, in several groups of C57/B1/6J mice produced a mean 33% loss of trabecular bone and a 140% increase in the area of bone lined by osteoclasts. Moreover, treatment of 4-month-old mice with di-M-PGE2 for 4 days caused a mean 15% depletion of bone calcium. There were no significant changes in blood calcium with the protocol employed.
No takes yet. Share an insight, caveat, or question.
Santoro et al. (1977) studied this question.