Put the two together: Physiologically relevant glycoproteins containing saccharides at predetermined sites, such as GlyCAM-1, can be generated from small synthetic and bacterially derived fragments by using the expressed protein ligation methodology. This technique was used for the synthesis of three semisynthetic GlyCAM-1 variants 1–3 (see scheme).
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Macmillan et al. (2004) studied this question.
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