The stimulatory action of TSH on 14C-leucine and 14C-tyrosine incorporation into protein by isolated bovine thyroid cells was investigated. When the cells were preincubated with actinomycin D so that RNA synthesis was severely inhibited, the TSH stimulation of protein synthesis was not greatly impaired. Hence, this action of TSH is not dependent on increased template RNA synthesis. Basal and TSH-stimulated levels of 14C-leucine incorporation could be depressed by omission of exogenous glucose. Furthermore, basal 14C-leucine incorporation was severely depressed and TSH stimulation was abolished when endogenous glucose metabolism was inhibited with 2-deoxyglucose. Therefore, it is concluded that the TSH effect on protein synthesis could be secondary to its stimulation of glucose metabolism. (Endocrinology80: 1101, 1967)
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Winton Tong (1967) studied this question.