Desiccation Stress Causes Structural and Ultrastructural Alterations in the Aeroterrestrial Green Alga Klebsormidium Crenulatum (Klebsormidiophyceae, Streptophyta) Isolated From an Alpine Soil Crust1

Klebsormidium crenulatum (Kütz.) Lokhorst (Klebsormidiophyceae, Streptophyta) isolated from an alpine soil in Tyrol, Austria, was experimentally exposed to desiccation under various relative air humidities (RH 5, 75, and >95%, ambient air 55%–60%). The effects on the structure and ultrastructure of K. crenulatum after 1, 4, or 7 d of desiccation at 5, 75, and >95% RH were investigated. The cross walls were deformed to an undulated shape, and the cell diameter was reduced to ∼60% of the control. Regardless of the RH applied, in all cases the cytoplasm appeared denser compared to that of liquid-culture-grown cells. Electron-dense particles with diameters of 0.4 μm–0.8 μm were observed in the cytoplasm, likely representing lipid droplets. The chloroplasts of desiccated samples contained a large number of plastoglobules. The number and appearance of mitochondria were not visibly altered, as also verified by 3,3′ dihexyloxacarbocyanine iodine (DIOC6) staining. The amphiphilic styryl dye FM 1-43 resulted in staining of the plasma membrane in cells from liquid culture. In 7 d desiccated samples, a marked fluorescence is seen in ∼40%–50% of the cells, which were dead. Actin microfilaments (MFs) were drastically disrupted after desiccation; only dotlike actin batches remained. These results demonstrate that flexibility of the cell walls and maintenance of the key organelles play a key role in the tolerance of desiccation stress in K. crenulatum.