Inducing and Sensing Protein–Protein Interactions in Living Cells by Selective Cross‐linking

Cells technique: Small molecules have been synthesized that enable the covalent and irreversible dimerization of fusion proteins of O6-alkylguanine-DNA alkyltransferase (AGT or SNAP-Tag) in vitro and in living cells. The cross-linking efficiency of AGT fusion proteins provides a measure to characterize the proximity and interactions of protein pairs in living cells (see scheme).