Quantification of phototrophic organisms on solid substrata together with their metabolic activity can be assessed easily, reliably and quickly through measurement of the organisms' colour. For that purpose only a chroma meter for solid substrata able to quantify the three components of colour is needed. A correlation between these three components and the number of organisms and their physiological state was demonstrated. The methodology developed here makes it possible to save time and materials in comparison with traditional microbiological methods. Moreover, it is a non-destructive method which can be used directly on site and in site. This characteristic is important when microbial environmental monitoring of cultural heritage is involved.
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Prieto et al. (2002) studied this question.
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