Treatment with 2.6 M urea of the Photosystem II particles depleted of two polypeptides of 24 kDa and 18 kDa completely released a polypeptide of 33 kDa and eliminated the oxygen‐evolution activity. The 33‐kDa polypeptide rebound to the urea‐treated particles and partially reactivated the oxygen evolution. A quantitative analysis of the rebinding suggests tha there is a specific binding site for the 33‐kDa polypeptide on the membrane surface.
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Miyao et al. (1983) studied this question.
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