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Iron, titanium, and vanadium analysis were performed on the tunicates Pyura chilensis Molina, 1782, and Ascidia dispar, and the inorganic chemistry of blood was investigated. The major ionic characterization of the blood plasma and cytosolic solutions were determined. Gel chromatography was used to secure information on the possible existence of metal organic complexes.Pyura chilensis accumulates Fe and Ti, and Ascidia dispar accumulates Fe, Ti, and V in blood cells in this quantitative order. Significant levels of metals are associated with cell residues (membrane cells), although this may be, to some extent, dependent on the cell lysis technique.The elution behavior of plasma in Sephadex G-75 and LH-20 gels and the respective absorption spectra of the fractions showed evidence of organic metal complexes in the plasma of both tunicate species.
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ROMAN et al. (1988) studied this question.
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