A mating scheme using crossover suppressors and dominant mutant markers was used to test chromosomes 2,4, and 5 for the presence of a postulated female determination factor (F) in the house fly, Musca domestica L. The data were negative for tests with chromosomes 2 and 5, but positive results were obtained for the presence of F on chromosome 4. Subsequent mapping showed that F was closely linked to the Ba (Bald abdomen, dominant) locus on chromosome 4.
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McDonald et al. (1978) studied this question.