The nutrition of seeding roots of Brassica rapa var. silvestris f. campestris «Stielmus» was manipulated to test whether sex determination in the beet cyst nematode, Heterodera schachtii, is controlled by environmental or genetic factors. Single J2 juveniles were added to germinated roots in water agar drops under aseptic condition. Half of the test plants were decapitated by removing root tips and shoots. Two days after adding the nematodes, nutrient solution containing minerals and various concentrations of sucrose was poured over the agar drops (...)
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Florian M. W. Grundler (1991) studied this question.
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