Functional characterization of the mucus barrier on the Xenopus tropicalis skin surface

Significance The production of mucus helps to trap pathogens, preventing their entry into the body, while it also acts as an interface for many important physiological events (e.g., gas and nutrient exchange). In mammalian models, a detailed study of mucus and its component parts is hindered by the difficulty in accessing these internally located tissues. The Xenopus tropicalis tadpole skin offers a complementary nonmammalian model system to study mucosal epithelia. Using this, we identify a mucin, similar to human mucins, that protects against infection. This system offers an experimentally tractable approach to study mucins and the mucus barrier and their role in conferring protection at mucosal surfaces.