Key result
5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate (GNHP) initiated T7 RNAP-promoted transcriptions to yield 5'-H2N-RNA that can be conjugated to activated esters.
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5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5'-H2N-RNA that can be conjugated to activated esters.
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GNHP can be synthesized and used to initiate T7 RNAP-promoted transcriptions, producing 5'-H2N-RNA suitable for conjugation.
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Williamson et al. (2007) studied this question. 5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate (GNHP) initiated T7 RNAP-promoted transcriptions to yield 5'-H2N-RNA that can be conjugated to activated esters.
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