Biliary Excretion by the Rat of Bromsulfalein as a Conjugate of Glycine and Glutamic Acid

Paper chromatograms of bile collected after the intravenous administration of bromsulfalein to rats reveal four distinct bromsulfalein bands. One migrates with the same Rf as standard bromsulfalein; the other three move less fast. The major band, which accounts for 71.3 percent of the injected bromsulfalein, appears to be a conjugate of bromsulfalein with glycine and glutamic acid.