A PCR‐based method for discriminating between high molecular weight glutenin subunits Bx7 and Bx7* in Triticum aestivum L.

With 1 figure and 1 table Abstract The correct assignment of high molecular weight glutenin subunit variants is a key task in wheat breeding for quality. However, traditional analysis by protein electrophoresis is sometimes difficult and not very precise. This work describes a novel DNA marker for accurate discrimination between the Glu‐B1 locus subunits Bx7 and Bx7*. An analysis of 142 bread wheat varieties from different countries identified a considerable number of misclassified genotypes that could lead to incorrect conclusions in studies of the relationship between glutenin composition and wheat quality.