Partial Analysis of the Capsid Protein (VP1) of Human Sapovirus Isolated from Children with Diarrhoea in Rural Communities of South Africa

Figures

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  Figure 1. Schematic diagram of human sapovirus partial genomic organization and RT-PCR target regions. The diagram presents the most targeted RdRp/VP1 junction by RT-PCR pointed by a black arrow, and the location of primers targeting the complete VP1 segment shown by grey arrows on the binding location.

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  Figure 2. Phylogenetic analysis of the partial polyprotein of human SV-GI detected in Vhembe district (South Africa) and reference strains selected from the GenBank database. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. A bar scale representing a genetic distance scale. The phylogenetic tree was deduced by the maximum likelihood method and the Kimura 2-parameter model using MEGA 11 [20, 21], based on a 709-nucleotide sequence fragment of the polyprotein (a VP1 segment) showing relationships of SV strains. The porcine SV (MF766258) was selected as an outgroup strain.

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  Figure 3. Phylogenetic analysis of the partial polyprotein of human SV-GII detected in Vhembe district, South Africa, and reference strains selected from the GenBank database. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. A bar scale representing a genetic distance scale. The phylogenetic tree was deduced by the maximum likelihood method and the Kimura 2-parameter model using MEGA 11 [20, 21], based on a 644-nucleotide sequence fragment. The porcine SV (MF766258) was selected as an outgroup strain.