Separation and Purification of Angiotensin Converting Enzyme Inhibitory Peptides Derived from Goat`s Milk Casein Hydrolysates

Enzymes and reagentsFresh goat's milk (Saanen; Ovis aegagrus) was purchased from Korean Medi-R Co. and then defatted by ultrafiltration (Supra 25 K, Hanil Sci., Korea).The separation of goat's milk CN was performed according to the methods of Sofia and Malcate (2000).Trypsin (Bovine pancreas, activity 3.3 Anson units g -1 protein) and Neutrase 0.8 (Bacillus subtilis, activity 0.8 Anson units g -1 protein) were from Novo Nordisk A/S (Bagsvaerd, Denmark), Protease S (Bacillus stearothermophilis, activity 10,000 units g -1 protein) and Papain W-40 (Carica Separation and Purification of Angiotensin Converting Enzyme Inhibitory Peptides Derived from Goat's Milk Casein Hydrolysates