IN studying uric acid synthesis in avian tissues a reliable micro-method for the determination of uric acid is desirable. Folin's colorimetric method and its modifications which are fairly reliable for blood, and possibly for normal urine, are very unsatisfactory in presence of tissue extracts. The colour-producing reaction lacks specificity [Fujiwara and Kataoka, 1933] and the colour developed is not wholly proportional to uric acid concentration. A second method developed by Fosse et al. [1930] depends on the ultimate quantitative conversion of uric acid into urea and glyoxylic acid by the enzymes uricase and allantoinase. With the enzyme preparations at our disposal, however, the analysis takes too long (12 hours). We first tried to improve this enzymic method but finally abandoned it in favour of a similar but non-enzymic procedure.
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Edson et al. (1936) studied this question.