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[4α-3H]Cholesterol was prepared in three steps from 4-oxo-cholest-5-en-3β-yl acetate, which was conveniently prepared from cholesterol. Introduction of the 4α 3H label utilised NaB3 H4 as the source of tritium. Details of this synthesis and also the synthesis of [4β-3H]cholesterol are given. Procedures for quantitative estimation of the tritium label at the 4α and 4β positions are also described.
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Lockley et al. (1978) studied this question.
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