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A novel trisialoganglioside has been isolated from dogfish (Squalus acanthias) brain. From the results of gas chromatography-mass spectrometric analysis of methylated sugars, enzymatic hydrolysis, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance analysis, its structure was concluded to be (formula; see text) This is the first report of the occurrence of a ganglioside with 3 sialic acid residues separately linked to its gangliotetraosyl backbone.
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Nakamura et al. (1988) studied this question.
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