Key result
The PPV RAA-LFD assay detected PPV DNA within 15 minutes at 37 °C with high sensitivity (limits of 10^2 copies/μL plasmid) and specificity, offering a more sensitive and cost-saving alternative to PCR.
Why the study?
Porcine parvovirus infection causes SMEDI syndrome and imposes a global burden on the swine industry, making rapid and efficient detection methods crucial.
The RAA-LFD assay provides a rapid, sensitive, and specific alternative for PPV detection, particularly useful in resource-limited settings.
Rapid PPV detection tool for swine; leaves open field validation before routine adoption.
Porcine parvovirus (PPV) infection is the primary cause of SMEDI (stillbirth; mummification; embryonic death; infertility) syndrome, which is a global burden for the swine industry. Thus, it is crucial to establish a rapid and efficient detection method against PPV infection. In the present work, we developed a recombinase-aided amplification (RAA) assay, coupled with a lateral flow dipstick (LFD), to achieve an amplification of PPV DNA at 37 °C within 15 min. The detection limits of PPV RAA-LFD assay were 102 copies/μL recombinant plasmid pMD19-T-VP1, 6.38 × 10−7 ng/μL PPV DNA, and 10−1 TCID50/mL virus, respectively. This method was highly specific for PPV detection with no cross-reactivity for other swine pathogens. In contrast to polymerase chain reaction (PCR), the PPV RAA-LFD assay is more sensitive and cost-saving. Hence, the established PPV RAA-LFD assay provided an alternative for PPV detection, especially in resource-limited regions.
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He et al. (2021) studied Porcine parvovirus (PPV) infection. Recombinase-aided amplification (RAA) assay coupled with a lateral flow dipstick (LFD) vs. Polymerase chain reaction (PCR) was evaluated on Detection limits and specificity for PPV. The PPV RAA-LFD assay detected PPV DNA within 15 minutes at 37 °C with high sensitivity (limits of 10^2 copies/μL plasmid) and specificity, offering a more sensitive and cost-saving alternative to PCR.
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