Key result
A monoclonal antibody (3H11) specifically reacting with the Cap protein of PCV2 was successfully developed in female BALB/c mice, without cross-reacting with PCV1.
The developed monoclonal antibody 3H11 specifically targets the PCV2 Cap protein without cross-reacting with PCV1, providing a useful diagnostic reagent for PCV2 research.
Female BALB/c mice were immunized with a commercial PCV2 vaccine, and a monoclonal antibody (MAb) designated as 3H11 was achieved by hybridoma techniques. The MAb specifically reacted with Cap protein of PCV2, which has been identified by western blot. Immunoperoxidase monolayer assay results showed that 3H11 did not cross-react with PCV1-infected cells. Therefore, this work suggested that 3H11 could be a useful tool as a specific diagnostic reagent for PCV2 research.
No takes yet. Share an insight, caveat, or question.
Gu et al. (2016) studied Porcine Circovirus2 (PCV2). Commercial PCV2 vaccine was evaluated on Development and characterization of a monoclonal antibody (3H11) against PCV2 Cap protein. A monoclonal antibody (3H11) specifically reacting with the Cap protein of PCV2 was successfully developed in female BALB/c mice, without cross-reacting with PCV1.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: