Key result
A novel multiplex PCR assay with a limit of detection of 10^2 copies/µl successfully identified PCV2, PPV, and ASF in porcine tissue samples, including a 1.40% triple co-infection rate.
Why the study?
A diagnostic method for simultaneously detecting and distinguishing African Swine Fever, porcine circovirus type 2, and porcine parvovirus in clinical specimens is critical for differential diagnosis, monitoring, and control.
Cross-Sectional (n=356)
A newly developed multiplex PCR assay can accurately and simultaneously detect ASF, PCV2, and PPV in porcine clinical samples.
Multiplex PCR enables simultaneous ASF/PCV2/PPV detection in pigs; leaves open field validation before routine use.
A diagnostic method for simultaneously detecting and distinguishing African Swine Fever (ASF), porcine circovirus type 2 (PCV2), and porcine parvovirus (PPV) in clinical specimens is critical for differential diagnosis, monitoring, and control in the field. Three primer pairs were designed and used to create a multiplex PCR assay. In addition, 356 porcine post mortem tissue samples from various parts of India's North Eastern region were tested by the developed multiplex PCR assay to demonstrate its accuracy. Using the designed primers, each of the ASF, PCV2 and PPV target genes was amplified, but no other porcine virus genes were detected. The assay's limit of detection was 102 copies/µl of PCV2, PPV, or ASFV. The detection of PCV2, PPV, and ASF in postmortem tissue samples revealed that they are co-circulating in India's North-Eastern region. The percentage positivity (PP) for PCV2, PPV and ASF single infection were 7.02% (25/356), 3.93% (14/356), and 3.37% (12/356), respectively, while the PP for PCV2& PPV co-infection was 2.80% (10/356), ASF & PCV2 co infection was 1.4% (5/356) and the ASF, PPV& PCV2 co-infection was1.40% (5/356). The results also indicate that the ASF can infect pigs alongside PCV and PPV.
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Pegu et al. (2022) conducted a cross-sectional in African Swine Fever, porcine circovirus type 2, and porcine parvovirus infection (n=356). Multiplex PCR assay was evaluated on Detection of PCV2, PPV, and ASF. A novel multiplex PCR assay with a limit of detection of 10^2 copies/µl successfully identified PCV2, PPV, and ASF in porcine tissue samples, including a 1.40% triple co-infection rate.
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