The application of the statistical study in the morphometric characterization of a colombian strain ofTrypanosoma cruzi

After morphological and histopathological characterization, the Cali strain of T. cruzi isolated from a patient in Cali (Colombia), was also submitted to a morphometrical characterization. For this we estimated 16 cellular parameters in 30 samples of each evolutive form of T. cruzi in blood in NNN medium through planimetry by the IBAS-1 (Image Basic Analytic System). This morphometrical study was completed with a statistical one through the application of the variance (ANOVA-1). This made it possible to evidence a great number of significant differences between the slender, broad and stout forms and also between the short and large epimastigotes from the NNN medium. These data have a real interest for classifying some features of the biological cycle of T. cruzi as well as the future development of diagnosis techniques or therapeutical and prophylactic experiments.