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Suspensions and dried preparations of vaccinia virus were exposed to γ-rays in cobalt-60 sources. Suspensions completely inactivated by 6–8 × 105rad. retained measurable immunogenicity. Dried preparations could not be completely in-activated even by doses of 11 × 106rad., although the inactivation apparently followed first-order kinetics until about one particle in 107survived, suggesting that the populations of vaccinia virus in the preparations were heterogeneous.
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Colin Kaplan (1960) studied this question.
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