Translation of Clover Yellow Mosaic Virus RNA in Pea Mesophyll Protoplasts and Rabbit Reticulocyte Lysate

Summary RNA from clover yellow mosaic virus, a potexvirus, was translated in rabbit reticulocyte lysates, and in vitro translation products were compared with virus-specific proteins produced in vivo in pea mesophyll protoplasts inoculated with viral RNA. One major polypeptide with an M r of 182000 (182K) was produced in vitro, and two were produced in vivo, with M r 182K and 22K. The 182K product was thought to be the same in vitro as in vivo; the 22K product was shown by Western blotting to be the virus coat protein.