Determination of seventeen s-triazine herbicides and derivatives by high-pressure liquid chromatography

Mixtures of simazine, atrazine, ametryne, prometryne, deethylsimazine, deethylatrazine, hydroxysimazine, hydoxyatrazine, hydoxyprometryne, N-ethyl- and N-isopropylammeline, N-ethyl- and N-isopropylammelide, melamine, ammeline, ammelide, and cyanuric acid in aqueous solution were separated and determined in a single analysis with a detection limit of 30-400 pmol. The s-triazines were detected by a UV detector after elution from a reversed-phase high-pressure liquid chromatography column using a phosphate buffer-methanol gradient at 2°C.