A microhemadsorption method is described for measuring the interaction between concanavalin A and single transformed or untransformed cells without removing them from the substrate. When indicator red blood cells were coated with Con A, their adherence to SV3T3 cells was only 2-4-fold greater than to 3T3 cells. On the other hand, when the two cell lines were coated with Con A, there was a very marked difference in the ability of the lectin on each to adsorb uncoated red cells.
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Furmanski et al. (1972) studied this question.