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It is known from previous work that macrocysts are formed when the two strains of Dictyostelium discoideum (NC4 and V12) are mixed. By culturing NC4 and V12 amoebae together in various ratios and at several densities, and in experiments using vitally stained cells, we have shown that the number of macrocysts formed is directly related to the number of V 12 (but not NC4) cells present and that macrocysts may occur without sexual fusion.
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Bozzone et al. (1982) studied this question.
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