An Investigation of Photo‐refractoriness in the House Sparrow by Artificial Photoperiods

Summary The natural testis cycle and associated changes in bill colour of the House Sparrow Passer domesticus are described. Subjects were wild‐caught at three different times in the breeding cycles:in February when the testes were small and contained only spermatogonia, in May when they were fully recrudesced and contained bunches of spermatozoa and in late July at a time when spontaneous gonad regression was occurring as the birds entered their photo‐refractory phase. Each sample was divided into eight separate groups which were given a range of artificial asymmetric skeleton photo‐periods generalized as LD 6 +1/2:131/2; the 1/2‐h light pulse was given at a different point in the dark period in each group. The birds entrained to skeleton schedules simulating complete photoperiods of up to 12 h duration as if the start of the long light pulse was dawn. However, with long‐day simulations (e.g. 6L 111/2 D 1/2 L 6D which simulates an 18 h photoperiod) the birds entrained to the start of the short light pulse to give an effective photoperiod of 121/2 h (that is, 1/2L 6D 6L 111/2D). The skeleton schedules simulating 14 h and 16 h were interpreted as such in February and were not effective in causing testicular induction, but they were apparently read as days of 161/2 h and 141/2 h in May, and as such maintained active spermatogenesis. The differences are discussed in terms of the changed frequency of a circadian oscillator. When birds captured in July were held on the various skeleton light schedules refractoriness was ended for most the subjects, as was proved by subsequently exposing them to a complete 16‐h photoperiod. Exceptions were the birds in the group given the 14‐h simulation, for these entrained as they had in May and so were effectively receiving a long 161/2‐h day. The discussion presents evidence to show that photoperiods in excess of 14 h cause the light sensitive phase of a circadian oscillator responsible for luteinizing hormone secretion to be phased into the non‐inductive period of darkness. Rehabilitation of the interstitial components of the testis is apparently inhibited in such circumstances.