An improved method of estimating serum calcium by the AutoAnalyzer using the reagent glyoxal-bis-(2-hydroxyanil) is described. Sensitivity and accuracy in the physiological range are increased by the use of ethylene diamine tetraacetic acid. Results correlate well with an established manual procedure and there is no interference from magnesium or phosphate.
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R G Burr (1969) studied this question.
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