Use of microautoradiography and fluorescent in situ hybridization for characterization of microbial activity in activated sludge

Research Article| January 01 1999 Use of microautoradiography and fluorescent in situ hybridization for characterization of microbial activity in activated sludge P. H. Nielsen; P. H. Nielsen *Environmental Engineering Laboratory, Aalborg University, Sohngaardsholmsvej 57, DK-9000 Aalborg, Denmark Search for other works by this author on: This Site PubMed Google Scholar K. Andreasen; K. Andreasen **Krüger A/S, Klamsagervej 2-4, DK-8230 Åbyhøj, Denmark Search for other works by this author on: This Site PubMed Google Scholar N. Lee; N. Lee *Environmental Engineering Laboratory, Aalborg University, Sohngaardsholmsvej 57, DK-9000 Aalborg, Denmark***Lehrstuhl Für Mikrobiologie, Technische Universität München, Arcisstr. 16, D-80290 Munich 19, Germany Search for other works by this author on: This Site PubMed Google Scholar M. Wagner M. Wagner ***Lehrstuhl Für Mikrobiologie, Technische Universität München, Arcisstr. 16, D-80290 Munich 19, Germany Search for other works by this author on: This Site PubMed Google Scholar Water Sci Technol (1999) 39 (1): 1–9. https://doi.org/10.2166/wst.1999.0001 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Share Icon Share Twitter LinkedIn Tools Icon Tools Cite Icon Cite Permissions Search Site Search nav search search input Search input auto suggest search filter All ContentAll JournalsThis Journal Search Advanced Search Citation P. H. Nielsen, K. Andreasen, N. Lee, M. Wagner; Use of microautoradiography and fluorescent in situ hybridization for characterization of microbial activity in activated sludge. Water Sci Technol 1 January 1999; 39 (1): 1–9. doi: https://doi.org/10.2166/wst.1999.0001 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex In order to understand the function of the different microorganisms in activated sludge, it is important to know their identity, diversity and activity. In this paper we describe how microautoradiography (MAR) and fluorescent in situ hybridization (FISH) can be used to characterize and enumerate functionally important groups of microorganisms in activated sludge. The principles for the methods, some important methodological aspects and limitations of the procedures and some important results are discussed. Special focus is on the examination of the in situ physiology of certain filamentous microorganisms and a discussion about the use of such information in mathematical models of the activated sludge processes. Enumeration, fluorescent in situ hybridization, in situ physiology, microautoradiography, 16S rRNA probes This content is only available as a PDF.