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G. affinis is the only species of the genus Gambusia positively identified from Australia. Meristic characters and gonopodium morphology of specimens from 22 localities throughout Australia were examined. Both support the identification of all specimens as the subspecies G. a. holbrooki. Previous reports indicating the presence of G. a. afinis or G. dominieensis in Australia are considered incorrect.
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Lloyd et al. (1985) studied this question.