Problem statement: Our aim was to enhance the data on antigenic properties of dromedary whey proteins.Approach: The identification of the whey proteins was carried by SDS-page and Reversed phase high performance liquid chromatography (RP-HPLC).The cross-reactivity of dromedary whey proteins with IgG anti bovine β-lactoglobulin and anti bovine α-lactalbumin, obtained by immunisation of Balb/c mice, was carried out by ELISA.Results: The SDS-page showed the presence of band corresponding to α-lactalbumin and albumin serum; this was confirmed by the chromatogram obtained by RP-HPLC, where we detected a pick corresponding to α-lactalbumin.There was a cross reaction of dromedary whey proteins with IgG anti bovin α- lactalbumin but it was very weak with IgG anti boin β-lactoglobulin.Conclusion: We detected a cross reactivity between dromedary whey proteins and IgG anti bovin α-lactalbumin.
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Youcef et al. (2009) studied this question.