Key result
Sodium butyrate at 1 mM increased the synthesis of recombinant human prolactin in CHO cells without compromising its structure or function.
Sodium butyrate at 1 mM enhances the synthesis of recombinant human prolactin in CHO cells without altering its bioactivity.
May boost CHO-based recombinant prolactin yields; leaves open scale-up and clinical manufacturing relevance.
Sodium butyrate (NaBu) has been used as a productivity enhancer for the synthesis of recombinant proteins in Chinese hamster ovary (CHO) cells. Thus, the influence of NaBu on the production of recombinant human prolactin (hPRL) from CHO cells was investigated for the first time. CHO cell cultures were submitted to a treatment with different concentrations of NaBu (0.25 to 4 mM). Quantitative and qualitative analyses by reverse-phase high-performance liquid chromatography (RP-HPLC) and Western blot or SDS-PAGE, carried out directly on CHO-conditioned medium, showed that the highest hPRL expression was obtained with 1 mM NaBu. In vitro biological assays based on noble rat lymphoma (Nb2) and mouse pro-B lymphoma (Ba/F3-LLP) cells were carried out on purified hPRL. Its bioactivity in the presence of NaBu was not apparently different from that of the First International Reference Reagent of recombinant hPRL (WHO 97/714). Our results show that NaBu increased the synthesis of recombinant hPRL in CHO cells, apparently without compromising either its structure or function.
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Goulart et al. (2010) studied this question. Sodium butyrate (NaBu) was evaluated on Production of recombinant human prolactin (hPRL). Sodium butyrate at 1 mM increased the synthesis of recombinant human prolactin in CHO cells without compromising its structure or function.
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