Cl−currents activated via purinergic receptors inXenopusfollicles

Ionic currents elicited via purinergic receptors located in the membrane of Xenopus follicles were studied using electrophysiological techniques. Follicles responded to ATP-activating inward currents with a fast time course (F(in)). In Ringer solution, reversal potential (Erev) of F(in) was -22 mV, which did not change with external substitutions of Na- or K+, whereas solutions containing 50 or 5% of normal Cl- concentration shifted Erev to about +4 and +60 mV, respectively, and decreased F(in) amplitude, indicating that F(in) was carried by Cl-.F(in) had an onset delay of approximately 400 ms, measured by application of a brief jet of ATP from a micropipette positioned near the follicle (50 microns). F(in) was inhibited by 50% in follicles pretreated with pertussis toxin. This suggests a G protein-mediated receptor channel pathway. F(in) was mimicked by 2-MeSATP and UTP, the potency order (half-maximal effective concentration) was 2-MeSATP (194 nM) > UTP (454 nM) > ATP (1,086 nM). All agonists generated Cl- currents and displayed cross-inhibition on the others. F(in) activation by acetylcholine also cross-inhibited F(in)-ATP responses, suggesting that all act on a common channel-activation pathway.