Characterization of Lactoferrin (LF) from Colostral Whey Using Anti‐LF Antibody Immunoaffinity Chromatography

Lactoferrin (LF) in colostral whey was isolated by anti‐LF immunoglobulin in yolk (IgY)‐Sepharose 4B immunoaffinity chromatography, and parameters such as binding capacity (q m ) and dissociation constant (K d , × 10 −6 M) of this immunoaffinity gel for LF were discussed. Purification folds for colostral whey I (from colostrum collected within 6 d of postpartum) and colostral whey II (from colostrum collected within 1 d of postpartum) by anti‐LF IgY‐immunoaffinity chromatography were 135.80 and 103.60, respectively. The recovery for LF in the same colostral whey sample by anti‐LF IgY‐immunoaffinity chromatography was 82 to 99 %. q m of anti‐LF IgY‐immunoaffinity gel for LF in colostral whey I and whey II were 0.372 and 0.272 mg LF/mL wet gel, respectively. K d of anti‐LF IgY‐immunoaffinity gel for LF in colostral whey I was 1.594 × 10 −6 M and II was 1.587 × 10 −6 M.