Temporal Separation of Transcription and Translation in Neurospora

Cells of Neurospora accumulated capacity for synthesis of kynureninase in the presence of cycloheximide and a suitable inducer. The capacity was expressed only when the cells were returned to a medium which allowed net synthesis of protein to resume. Subsequent expression did not require synthesis of ribonucleic acid (i.e., was not inhibited by actinomycin D) or the presence of inducer. The relative level of capacity, as judged by the initial rate of appearance of enzyme, increased linearly for 70 min in cells incubated in the presence of inducer plus antibiotic.