Calmodulin binding to nitrocellulose and Zetapore membranes during electrophoretic transfer from polyacrylamide gels

In the classical electrotransfer conditions from sodium dodecyl sulfate‐polyacrylamide gels, calmodulin fails to bind to nitrocellulose membranes. However, under conditions favorizing hydrophobic interactions such as a higher salt concentration, calmodulin can be immobilized on nitrocellulose sheets, thus allowing detection in a complex protein mixture.